Automated physiological analysis of Engineered Heart Tissues from Human Induced Pluripotent Stem Cells
Presented by Eli Lefkowitz, Catalina Foothills High School
Cardiovascular disease is responsible for one in every four deaths. To understand these deaths, cardiomyocytes can be generated from a patient’s blood by first converting blood into a stem cell state called induced pluripotent stem cells (iPSCs). Cardiomyocyte derived iPSCs (hiPSC-CM) can be assembled into 3D constructs that resemble the heart called engineered heart tissues (EHTs). While it is possible to perform cardiovascular disease modeling in a dish, the tools to analyze the function of hiPSC-CMs remains laborious. To meet this need, our lab developed a software (MATLAB based) to quantify cardiac physiological parameters (peak amplitude, peak duration, and faster rising rate) in an automated and highly accurate manner. Further deployment of our software will be used to understand how mutations lead to cardiovascular disease.