Swapnika Raola

Swapnika Raola, University of Southern California (Undergraduate Student)

End-point PCR Analysis Using Capillary Flow as an Alternative to Gel Electrophoresis

We are finding a faster alternative to gel electrophoresis for template DNA identification. Using the same primer, bacteria strains Escherichia coli K-12 and O157:H7 form different amplicon lengths. We believe that amplicon length can affect capillary flow through interfacial tension.

Thus, we ask, “Does differing amplicon length and interfacial tension affect the capillary flow of  escherichia coli K-12 and Escherichia coli O157:H7 DNA solution?” If so, bacteria can now be identified through analysis of capillary flow, a process that takes two minutes rather than gel electrophoresis’s two hours. We found there are significant differences in capillary flow between the two strains and found that interfacial tension is probably the mechanism. Therefore, researchers can use our capillary flow model as a faster alternative to gel electrophoresis.